PhD Position: "Sex-bias in the regulation and function of R-loops" (m/f/d)

The research group of Maria Felicia Basilicata offers the following PhD project:

Neurological autoimmune disorders occur when the body's defense system mistakenly attacks healthy brain tissue. This group includes conditions like Multiple Sclerosis (MS) and some types of Systemic Lupus Erythematosus (SLE) that affect the nervous system. At the molecular level, these disorders involve complex interactions between the immune system and the central nervous system. A key factor is the overactivation of type 1 interferon, a part of the immune system that recruits immune cells to the central nervous system. This persistent process results in chronic inflammation and tissue damage. At the molecular level, the aberrant formation of RNA-DNA hybrids known as R-loops has been implicated in activating innate responses. These DNA RNA hybrid structures can form during transcription in brain cells and may contribute to genomic instability and altered gene expression. A striking feature shared by most autoimmune diseases is a strong female predominance, meaning women are much more likely to develop these disorders than men. The reasons for this disparity are not fully understood and remain an active area of research.

Interestingly, dietary interventions have shown promise in managing these conditions. The ketogenic diet induces a metabolic shift in the body. Originally developed as a therapeutic approach for epilepsy, this diet has recently proven beneficial in the management of Multiple Sclerosis. The mechanisms underlying these effects are still being elucidated but may involve sex specific modulation of neuroinflammation and neuroprotection.

PhD Project: Implications of selective escape from X-inactivation in neurodevelopmental disorders

We are seeking an enthusiastic researcher to join our young team exploring sex bias and environmental triggers in neuronal vulnerability. You will investigate the interplay between R-loop accumulation, sex-specific brain dynamics, and metabolic factors in female-biased neurological autoimmune disorders.

You will gain hands-on experience with: Primary cell cultures (human/murine), 2D and 3D neuronal co-cultures, CRISPR/Cas9 engineering, Fluorescence-based imaging and analysis, Multiple Omics assays (e.g. RNAseq, CUT&Tag/RUN, DRIP). We offer a supportive environment, state-of-the-art facilities, and opportunities for interdisciplinary collaboration.

Our ideal Candidate is enthusiastic and an open-minded individual who:

• Shows curiosity and passion for understanding complex biological processes from molecular to organismal level.
• Has strong will to collaborate, work in a team and contribute to positive lab culture
• Enjoys discussing both basic and translational aspects of science.
• Previous experience with immunology and/or bioinformatics is a plus.

If you are interested in this project, please select Basilicata as your group preference in the IPP application platform.

Are you an ambitious, young scientist looking to push the boundaries of research while interacting with colleagues from multiple disciplines and cultures? Then joining the IPP is your opportunity to give your scientific career a flying start!
All you need is:

• Master or equivalent
• Interactive personality & good command of English
• 2 letters of reference

• Exciting, interdisciplinary projects in a lively international environment, with English as our working language
• Advanced training in scientific techniques and professional skills
• Access to our state-of-the-art Core Facilities and their technical expertise
• Fully funded positions with financing until the completion of your thesis
• A lively community of more than 200 PhD students from 44 different countries

For more details on the projects offered and how to apply via the "Apply Now" Button.

The deadline for applications is 24 October 2024. Interviews will take place at IMB in Mainz on 20-22 January 2025.
Starting date: 1 March 2025 - 1 July 2025