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Master Thesis in Biotechnology / Process Engineering / Technical Biology

Master Thesis in Biotechnology / Process Engineering / Technical Biology
“Kinetic Characterization of an in-vitro Enzymatic Cascade for Nucleotide Sugar Production by state-of-the-art Ion Exchange Chromatography”

Glycosylation is an important modification of proteins, and is involved in the regulation of biological activity and stability of many proteins. Notably, the glycosylation of many eukaryotic proteins appears to be highly-regulated and minor structural changes of glycan portions may lead to enzyme dysfunction. Research into glycosylation has, thus, profoundly increased over the last years and controlling protein glycosylation is seen as a key for the cost-effective manufacturing of biotechnological products. In a joint project with the University of Halle-Wittenberg, an in-vitro enzyme cascade consisting of endoplasmatic reticulum glycosyltransferases is build up to produce Nglycans enzymatically. Glycosyltransferases require nucleotide sugar-donors (e.g. GDP-mannose), which are highly priced chemicals that need to be produced enzymatically as well for an economically efficient production process. Thus, the proposed master project will focus on the kinetic characterization and optimisation of the enzymatic GDP-mannose production. The kinetic constants are determined by ion exchange chromatography.

Determination of the kinetics and optimum conditions of a novel enzyme cascade for
nucleotide sugar production by state-of-the-art ion exchange chromatography

- Background in biotechnology, bioengineering, biosystems technology, process engineering, biochemistry, analytical chemistry or related fields
- Ideally, experience with ion exchange chromatography and enzyme kinetics
- MS Office, ability to work in a team

We offer
- Participation in research and development of a key technology
- Excellent supervision and thorough training by our staff
- Cutting-edge equipment and technology

March/April 2015


Contact detail

How to apply:
Only by Email!
Send application to
Dr. Thomas Rexer
E-Mail: rexer@mpi-magdeburg.mpg.de
Phone: +49 (0) 391 6110 375

Job profile

Working hours
Can be Full-Time or Part-Time
Contract duration
Type of job
Diploma Thesis/Master's Thesis
Work experience
job experience is not required
Germany (Sachsen-Anhalt)
Working place
39106 Magdeburg
Area of expertise
Biology & Life Sciences, Biotechnology, Chemical & Process Engineering