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PhD - Chromatin recruitment of the kinase HIPK2: mechanisms and physiological consequences

The Institute of Biochemistry (Medical Faculty) is inviting applications for a
PhD student (E13/2) in Epigenetics
for the project "Chromatin recruitment of the kinase HIPK2: mechanisms and physiological consequences". This project is part of the transregional collaborative research center (TRR81). The position is immediately available for 3 years.
The project:
This project will investigate the molecular mechanisms and physiological consequences of chromatin recruitment of the serine/threonine kinase HIPK2 . We have already performed ChIP-Seq experiments and found that HIPK2 binding motifs have a prominent overlap with DNA motifs for a zinc finger transcription factor, suggesting that this transcription factor functions as a docking port for the kinase. It is thus planned to measure chromatin association of HIPK2 or the transcription factor after shRNA-mediated depletion of the respective interaction partner. It will also be important to determine the effects of transcription factor and/or HIPK2 depletion on mRNA expression of their target genes by RT-qPCR. The transcription factor and HIPK2 share a number of common binding partners, therefore it will be interesting to reveal whether these partners also occur at the genomic loci containing the transcription factor/HIPK2 complex. In addition we will use the CRISPR-Cas system to remove prototypic genomic HIPK2/transcription factor binding sites, followed by the analysis of HIPK2 and transcription factor chromatin recruitment by ChIP experiments. A variety of different methods will be employed to understand the mechanisms and physiological consequences of HIPK2 chromatin recruitment.
Your profile: You have succesfully finished your Master or equivalent degree. You are highly self-motivated and ambitious and can work well in a highly active international research team, but also work on your own. Participation in the running  teaching program is expected.
We offer: The Institute (http://www.uni-giessen.de/cms/fbz/fb11/institute/biochemie/forschungbiochemie/agschmitz) offers the opportunity to work in an interactive and dynamic setting, is equipped with state-of-the-art facilities and well funded. We provide a creative working atmosphere and have active exchange programs with many scientists abroad.
The university is an equal opportunity employer, and applications from women or candidates with children are encouraged. Applications of severely disabled with same suitability are preferred. Application deadline is end of July 2014.
Please send your CV, two names of reference and the further usual documents to
Prof. M.L. Schmitz
Biochemical Institute
Friedrichstrasse 24
35392 Giessen (Germany)
e-Mail: lienhard.schmitz@biochemie.med.uni-giessen.de
We would appreciate your sending the application papers in copy, since we are not going to send your papers back once the application procedure is going to be finished.

Contact detail

How to apply:
Send application to
Prof. M.L. Schmitz
Biochemical Institute
Friedrichstrasse 24
35392 Giessen (Germany)
e-Mail: lienhard.schmitz@biochemie.med.uni-giessen.de

Job profile

Working hours
Contract duration
Type of job
PhD Project
Work experience
job experience is not required
Germany (Hessen)
Working place
35392 Giessen (Lahn)
Area of expertise
Biology & Life Sciences, Chemistry, Pharmaceutics