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PhD position in Molecular Biology - DNA demethylation

In the field of  “DNA demethylation” the research group of Prof. Christof Niehrs offers the following PhD project:

Characterisation of a new DNA demethylation pathway 

Description:
While the mechanisms of active DNA methylation are still incompletely understood, one process which seems universally involved in active DNA demethylation is DNA repair. We recently discovered a novel DNA demethylation repair activity in human cell extracts. The reaction involves a characteristic nucleotide repair pattern of unknown origin. We identified candidate proteins  as potential demethylation factors in this reaction. These findings may open up an exciting, novel avenue for mammalian DNA demethylation. The PhD project aims to systemically characterise the identified candidate proteins for their role in DNA demethylation in vitro and in vivo and, hence, to identify the underlying (repair) mechanism of the novel demethylation reaction. We will conduct loss- and gain-of-function experiments in cell culture and analyze the effect of candidates on global genome methylation by quantitative mass spectrometry and bisulfite sequencing. ChIP-sequencing of the candidate proteins will allow us to identify endogenous demethylation targets. We will knockout candidate genes in mouse embryonic stem cells (mESCs) by the CRISPR/Cas9 genome editing technique and analyze the physiological relevance of these genes in pluripotency and during mESC in vitro differentiation by e.g. RNA sequencing.  

We offer
•    The possibility to work on a cutting-edge project using state-of-the-art technology in a highly motivated research team
•    A stimulating, diverse and international research environment
•    Advanced training opportunities
•    A competitive stipend

Required qualifications
•    Master or Diploma
•    Motivation to solve complex biological problems
•    Excellent communication skills

Starting date: October 2015 or later
Duration of stipend: 3 years, with the possibility of extension
Deadline for registration (exclusively online via web form):  20 June 2015

How to apply:
https://www.imb-mainz.de/students-postdocs/international-phd-programme/apply-to-ipp/
Send application to
http://www.imb.de/PhD
While applying for the job please refer to jobvector and use the following reference number: JV-C8-CN2

About Institute of Molecular Biology gGmbH

Our International PhD Programme on the “Gene Regulation, Epigenetics and DNA Damage Response” gives talented and enthusiastic students the opportunity to undertake PhD research at the cutting edge of modern biology. Our groups cover a broad range of expertise and include leading biochemists, geneticists, cell and developmental biologists who study the molecular mechanisms of...

More about Institute of Molecular Biology gGmbH

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