Master Thesis: Production of influenza virus in high cell density MDCK suspension culture
Production of influenza virus in high cell density MDCK suspension culture
For the master thesis a MDCK suspension cell line will be cultivated to high cell densities (min. 40 Mio cells/mL) in different cultivation media. First a semi perfusion approach will be used for small scale cultivation in shaker flasks followed by a scale up to a 1 L STR with alternating flow filtration (ATF) or tangential flow filtration (TFF) for cell retention. Additionally, the infection of the MDCKsus cells will be optimized both in small scale and in a STR. For this project you will learn skills for the cultivation of mammalian suspension cells (shaker and STR), employ state of the art perfusion techniques for high cell density and use a wide set of analytical tools for cell cultivation (cell counter, metabolic analysis, online monitoring) as well as for virus infection (HA-assay, TCID-50, cytometer). You will work in a state of the art cell culture laboratory in an international group of researchers, TAs and students.
This announcement refers to students in the field of (bio)chemical engineering, biotechnology, technical biology, biosystems engineering or related fields with a passion for process optimization of biological systems. Experience in the field of cell culture, reactor cultivation or virology are beneficial. Self-directed initiative goal orientated work in the context of our research area is expected.
Please send your application (motivation letter, CV, high school & university grades and references) until December 31st, 2016 only via E-mail.
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About Max Planck Institute for Dynamics of Complex Technical Systems
In 1996 the Max Planck Institute for Dynamics of Complex Technical Systems was established. The foundation director is Prof. em. Dr.-Ing. Ernst Dieter Gilles. The institute in Magdeburg is the first institute of the Max Planck Society dedicated to the engineering sciences and is supposed to bridge the gap between basic research and industrial applications. The neighbourhood to the Otto...More about Max Planck Institute for Dynamics of Complex Technical Systems