PhD position in Genomic views of splicing regulation
Binding dynamics at the 3' splice site
The binding of U2AF65 to the polypyrimidine tract at the 3' splice site constitutes an important checkpoint of splicing regulation. The broad binding specificity of U2AF65 enables the recognition of a heterogeneous spectrum of polypyrimidine tracts, which in turn allows the interplay with multiple regulators. The polypyrimidine tract therefore constitutes a prime example of how protein binding to a cis-regulatory element is shaped by the interactive environment. Importantly, mutations in various factors that act during 3' splice-site definition have recently been implicated in the development of several haematological malignancies and have been identified as promising targets of anti-cancer drugs.
This project aims at a genomic understanding of U2AF65 activity in the context of its activators and repressors. The PhD student will combine state-of-the-art genome-wide in vivo RNA biology tools, such as RNA-seq and iCLIP, with biochemical and in vitro experiments. This will provide us with the first genome-wide view of how the environment in living cells shapes the recruitment of U2AF65 to nascent RNA. The project will rely on close collaborations with computational scientists, and an interest of the PhD student in bioinformatics approaches is beneficial.
• The possibility to work on a cutting-edge project using state-of-the-art technology in a highly motivated research team
• A stimulating, diverse and international research environment
• Advanced training opportunities
• A fully funded PhD position
• Master or Diploma
• Motivation to solve complex biological problems
• Excellent communication skills
Starting date: 1 March 2017 or later
Deadline for applications (exclusively online via web form): 10 November 2016
While applying for the job please refer to jobvector and use the following reference number: JV-C11-JK1
About Institute of Molecular Biology gGmbH
Our International PhD Programme on the “Gene Regulation, Epigenetics and DNA Damage Response” gives talented and enthusiastic students the opportunity to undertake PhD research at the cutting edge of modern biology. Our groups cover a broad range of expertise and include leading biochemists, geneticists, cell and developmental biologists who study the molecular mechanisms of...More about Institute of Molecular Biology gGmbH