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Analysis of the trehalose metabolism in Actinoplanes sp. SE50/110 with regard to acarbose production and byproduct formation

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Background
Actinoplanes spp. are Gram-positive aerobic bacteria producing a variety of pharmaceutically relevant substances. Since 1990 the α-glucosidase-inhibitor acarbose is produced and marketed for the treatment of type-2 diabetes mellitus. Actinoplanes sp. SE50/110 produces different acarviostatins depending on the C-source (Wendler et al. 2014) and various acarbose-like byproducts during fermentation. Wehmeier & Piepersberg described 2004 in particular the byproduct component C (acarviosine-trehalose) which is hard to separate from acarbose during downstream processing. It is therefore worthwhile to study the biosynthesis of trehalose in Actinoplanes sp. SE50/110 and to clarify its role in the production of component C.

Aims of the project
In 2008 Lee et al. postulated three putative trehalose synthetic pathways in Actinoplanes and suggested that one of these proteins, the maltooligosyltrehalose synthase TreY of the treXYZ gene cluster plays a role in the formation of component C. The goal of this Ph.D. project is now to elucidate in Actinoplanes sp. SE50/110 the complete machinery for trehalose biosynthesis. This can be achieved by a mutational analysis of trehalose biosynthesis genes. In addition, the transcriptional regulation of the trehalose biosynthesis genes should be analyzed. Furtheron, the influence of a mutated treXYZ gene cluster on the production of acarbose and the formation of component C shall be analyzed by applying metabolomics techniques.
 

Contact detail


How to apply:
Applications for fellowships with the CLIB-Graduate Cluster Industrial Biotechnology are centrally collected during the application call and distributed to the project leaders after the deadline. Please do not send your application by email. The application website will be open from 22 Oct - 15 Nov 2015. The application is a two-step process:
1. Online Application Form
  • Fill in and submit the online application form available at http://www.clib-graduatecluster.de/jobs/phdreg/apply/
  • Use Internet Explorer with cookies enabled to avoid technical difficulties.
  • You will receive an automated email with information on how to log in to upload your certificates in step 2.
  • 2. Certificates File

  • Prepare one pdf file with all your relevant certificates (for example your Masters degree, transcript of records, your secondary school leaving certificate, a brief CV, TOEFL etc.). Please include also a motivation letter.
Send application to
manBIE@graduatecluster.net

Job profile


Working hours
Full-Time
Contract duration
Temporary
Type of job
PhD Project
Work experience
job experience is not required
Region
Germany (Nordrhein-Westfalen)
Working place
33615 Bielefeld, NRW
Area of expertise
Biology & Life Sciences, Biotechnology