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Doctoral Researcher Position (Ref. No. ILRS_07/2016): The impact of redox regulation on the stress response of Aspergillus fumigatus and its role in host-pathogen interaction

The Leibniz Institute for Natural Product Research and Infection Biology – Hans Knöll Institute – in cooperation with the Friedrich Schiller University, the University Hospital Jena and the Max Planck Institute for Chemical Ecology are offering an international graduate training programme. The

International Leibniz Research School (ILRS Jena)

gives doctoral researchers the possibility to prepare for their PhD exam in an ambitious program providing excellent research conditions. We invite applications for a
 
Doctoral Researcher Position (Ref. No. ILRS_07/2016)
The impact of redox regulation on the stress response of Aspergillus fumigatus and its role in host-pathogen interaction

Reactive oxygen (ROS) and nitrogen species (RNI) are formed during normal metabolism of eukaryotic cells. The major source of ROS production is the mitochondrial respiratory chain. However, other sources of ROS have been reported, such as the oxidative folding within the lumen of the ER or by NADPH oxidases. RNI are produced by NO synthases or in hypoxic mitochondria. Besides being implicated in protein damage, recent studies suggest that ROS and RNI are regulatory agents of key cellular functions mediated primarily through reversible cysteine oxidation. More recent studies of our group have shown that the human pathogenic fungus Aspergillus fumigatus is exposed to higher intracellular oxidative stress during short-term adaptation to hypoxia, antifungal agents or confrontation with human neutrophilic granulocytes. The intracellular distribution of ROS and the sites of oxidative thiol modifications have not been identified in detail yet under the aforementioned stress conditions. In addition, the impact of cysteine oxidation on physiological processes of A. fumigatus is largely unexplored.

The proposed PhD project aims to establish redox biosensors for A. fumigatus based on different members of the green fluorescent protein family, which enable direct sensing of intracellular superoxide anions, hydrogen peroxide or the redox potential (glutathione) under specific stress conditions (hypoxia, exposure to antifungals, immune effector cells). These biosensors provide the advantage of being independent of cellular probe uptake or cell disruption and allow measuring in individual organelles of the cell. In a further step, quantitative redox proteomic methods will be established which allow the identification and characterization of oxidative posttranslational modifications (PTMs), such as protein thiol oxidation or S-nitrosylation. After having established the above described methods the changes of the intracellular redox status of A. fumigatus during interaction with human phagocytes will be investigated.

We expect:
  • a Master’s degree (or equivalent allowing you to pursue a PhD degree) in Natural or Life Sciences. Research at ILRS is centred around “Microbial and Biomolecular Interactions”. Candidates about to earn their degree are welcome to apply.
  • high motivation and interest to join one of the research areas of ILRS
  • creativity and interest in shaping your own thesis project
  • an integrative and cooperative personality with enthusiasm for actively participating in our lively community
  • very good communication skills in English
  • experience in molecular microbiology and biochemistry is required, knowledge and experience with proteomic methods is preferable
We offer:
  • a top-level research environment
  • efficient supervision by a team of advisors
  • a comprehensive mentoring programme
  • courses in state-of-the-art technologies and soft skills
  • strong communication and interaction between the involved institutions
  • Jena – City of Science: innovative business activities, successful scientific centres and a vibrant cultural scene around the famous Friedrich Schiller University
The three-year Doctoral Researcher position is available starting from January 2017. The position will be financially supported according to TV-L (salary agreement for public service employees). HKI is an equal opportunity employer.

Further information:
Dr. Olaf Kniemeyer, PI, olaf.kniemeyer@leibniz-hki.de
Dr. Christine Vogler, ILRS Coordinator, ilrs@leibniz-hki.de

The application process is handled exclusively online. Please acquaint yourself with the scientific projects offered on our website. Please note that you can choose up to 3 projects of interest in your application. It is only necessary to fill in the application form for your first preference of projects; the other two projects can be selected in the application form. In order to apply via the online application portal, go to

https://orthos.hki-jena.de/jobs/job-offers

Deadline for application: Oct 23, 2016.
Successful applicants will be invited to attend a recruitment meeting in Jena (envisaged date: Dec 14-15, 2016).
 

Contact detail


How to apply:
The application process is handled exclusively online. Please acquaint yourself with the scientific projects offered on our website. Please note that you can choose up to 3 projects of interest in your application. It is only necessary to fill in the application form for your first preference of projects; the other two projects can be selected in the application form. In order to apply via the online application portal, go to

https://orthos.hki-jena.de/jobs/job-offers
Send application to
Dr. Christine Vogler, ILRS Coordinator, ilrs@leibniz-hki.de
 

Job profile


Working hours
Full-Time
Contract duration
Temporary
Type of job
PhD Project
Work experience
job experience is not required
Region
Germany (Thüringen)
Working place
07745 Jena
Area of expertise
Biology & Life Sciences, Biotechnology