PhD position 10: Multifaceted functions of extracellular vesicles in a thrombus and in thrombo-inflammation (Marseille/Maastricht)

We offer a PhD position for biomedical doctoral students at the Center for CardioVascular and Nutrition research (C2VN at Aix-Marseille Université, France) and the School for Cardiovascular Disease in Maastricht (CARIM, Netherlands). As this project is part of the MSCA ITN “Thrombo-Inflammation in Cardiovascular Disease” (TICARDIO), you will be trained in an international, interdisciplinary joint doctoral degree program striving to train the next generation of biomedical researchers. Your PhD project will be supervised by Prof. Dr. Françoise Dignat-George in Marseille and by Dr. Rory Koenen in Mastricht. Furthermore, a research stay at BioCytex (Marseille, France, Dr. Philippe Poncelet) offers you the opportunity to get insights into research in the industry.

Activities and responsibilities

Project description
Extracellular vesicles (EV) are small, membrane-enclosed cell fragments. The hypothesis of this project is that besides the origin of EV, the activation state of the mother cells also determines their protein composition and function. This project focusses on EV formed at the site of a stable or unstable arterial thrombus.
You will study the formation and roles of EV in thrombus formation and thrombolysis using relevant mouse models. You will characterise endothelial cell (EC) derived EV, using a paramagnetic beads capture technology. You will examine thrombus formation and resolution in mice lacking two membrane phospholipid scrambling proteins. Thrombus size and organisation with EV will be determined in vivo with fluorescent labels and/or by histology and immunohistochemistry. You will apply the EV isolated at BioCytex to these mouse models and characterize their procoagulant and fibrinolytic properties. In the same mice, effects of infused EV on chemokine production and expression of inflammatory adhesion molecules by EC will be determined. You will distinguish the contributions of endogenous EV derived from EC, monocytes, neutrophils and platelets in thrombosis and thrombolysis, using appropriate tissue-specific knockouts mice. At CARIM, you will use established in vitro models to study mechanisms of coagulant and fibrinolytic properties of EV in interaction with EC and smooth muscle cells. As a translational extension, the biomarker potential of procoagulant EV will be assessed in plasma from patients with syndromic thrombosis.

Qualification profile

>> You have a Master degree (or equivalent) in biomedical sciences or a similar field
>> You are in the first four years of your research career and do not hold a doctoral degree
>> Your must not have resided in France for more than 12 months in the 3 years prior to the recruitment
>> Ideally, you have experience in cell culture and cell isolation from biologic specimen
>> Experience with flow cytometry is desirable

Benefits

Benefits of working in an ITN
>> You will be working within our international group of > 30 researchers
>> You will be involved in cutting-edge research in an interdisciplinary environment
>> You will contuct your research at two research locations and the site of an industry partner
>> You will receive excellent network-wide and local training with conference participation
>> You will receive a 36 months contract with all national social security (and family allowance, if applicable) at the Aix-Marseille Université.
>> You will optain a joint doctoral degree from Universities of Marseille and Maastricht, if all requirements are fullfilled

How to apply
Please visit www.ticardio.eu for information about the recruitment process and all required application documents.

Application deadline: July 15, 2019

Send application to

How to apply
Please visit www.ticardio.eu for information about the recruitment process and all required application documents.

While applying for the job please refer to jobvector and use the following reference number: JV-50056785

About Universitätsmedizin Mainz

Center for thrombosis and hemostasis The scientific activities of the researching physicians and scientists of the medical units of the Mainz University Medical Center are characterized by a broad content spectrum. In recent years, university medicine has been able to confirm its national and international position through a large number of excellent publications and the acquisition of...
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