We are very sorry but it looks like you are using an outdated browser. We strongly recommend updating to a modern browser.
More information about your browser and how to update

PhD position in stem cell technology, liver physiology and transcriptional regulatory networks

This job ad is out of date. Find related Jobs here.

The Leibniz Research Centre for Working Environment and Human Factors (IfADo) at the University of Dortmund integrates both basic and applied science in its mission to understand human health and disease.  Our strength is in our diversity as scientists from different academic backgrounds work together to address specific questions in the areas of liver and metabolic diseases, cancer development and progression, and stress and aging. The successful candidate will join an interdisciplinary network of molecular biologists, immunologists, bioinformaticians, systems biologists and physicians. Our group has a strong background in imaging with several possibilities for microscopy-based analyses. We also have ongoing collaborations for metabolic and lipid analyses.

PhD position in stem cell technology, liver physiology and transcriptional regulatory networks


The ability to generate mature hepatocytes from stem cells represents an enormous potential in medicine, pharmacology and toxicology. However, all current differentiation protocols achieve a hepatocyte-like cell state (HLCs) that does not faithfully recapitulate mature hepatocyte properties. To solve this problem, we will apply a gene network, systems biology model-based design for molecular interventions to improve hepatic differentiation of iPSCs. Recently, we identified a blue print for the most critical molecular networks controlling the differentiation of stem cells into hepatocyte-like cells (Godoy P. et al: Gene networks and transcription factor motifs defining the differentiation of stem cells into hepatocyte-like cells. J. Hepatol. 2015 Oct;63(4):934-42). Based on these findings, the successful applicant will implement an iterative experimental program of interventions in iPSC cultures including siRNA, cytokine/growth factors and ECM, with the aim of enhancing the hepatic differentiation of HLCs. The outcome of the interventions will be analysed by transcriptomics, epigenomics and signal transduction modelling in close cooperation with national and international experts in stem cell technology, transcriptomics and systems biology.    
Experience in stem cell differentiation, signal transduction (i.e. Western blot, bead-based ELISA) and transcriptional control mechanisms (real time quantitative PCR) are highly desirable. Experience in flow cytometry-cell sorting, basic molecular biology/cloning and generation of lentivirus vectors are also an asset.       
Our institute offers excellent research conditions, including well-equipped cell and molecular biology labs, confocal and two-photon based microscopy, flow cytometry facility, genome wide analyses, and a mouse facility with different reporter strains of liver cell types. For further information, please contact Dr. Patricio Godoy (email: godoy@ifado.de, Tel: +49-231-1084370). 

Contact detail


How to apply:
The position is immediately available. Please send your application with reference to the project, including a CV, a letter of motivation, and the name and contact information of two referees 
IFADO is an equal opportunity employer, and does not discriminate on gender, race, colour, religion, national origin, disability, or age in its programs and activities
Send application to
Dr. Patricio Godoy (email: godoy@ifado.de, Tel: +49-231-1084370).

or to

 Ms. Silke Hankinson
hankinson@ifado.de

or by post to the following address:

Leibniz-Institut für Arbeitsforschung
an der TU Dortmund (IFADO)
Ms. Silke Hankinson
Ardeystr. 67
D-44139 Dortmund
 

Job profile


Working hours
Part-Time
Contract duration
Temporary
Type of job
PhD Project
Work experience
jobexperience is required
Region
Germany (Nordrhein-Westfalen)
Working place
44139 Dortmund
Area of expertise
Biology & Life Sciences
APPLY NOW