PhD Thesis: Unravelling the role of CENP-A in DNA damage response

The highly organized processes of cell division that take place at centromeric chromatin during mitosis and meiosis are still not fully understood. However, it is well known that instead of centromeric DNA sequences, epigenetic mechanisms regulate the formation at kinetochores at centromeres. The best-characterized epigenetic mark for centromeres is the histone H3-variant CENP-A, which replaces H3 in some of the nucleosomes within centromeric chromatin. We have recently shown that CENP-A reacts to DNA damage induction and unpublished results suggest that posttranslational modifications of CENP-A and other centromere-associated proteins are important for DNA–damage induced changes. This project is designed to uncover the relevance of changes in chromatin upon genotoxic stress for kinetochore formation, mitotic progression and faithful chromosome segregation. The projects involve a fast variety of biochemical, molecular and cell biological techniques, as well as high resolution, super resolution and live cell microscopy.
Mathew, V., Pauleau, A. L., Steffen. N., Becker P.B., Erhardt, S. The histone-fold protein CHRAC14 influences chromatin composition in response to DNA damage. Cell Reports 7, 321-330, doi:10.1016/j.celrep.2014.03.008 (2014).

Bade, D., Pauleau, A. L., Wendler, A. & Erhardt, S. The E3 ligase CUL3/RDX controls centromere maintenance by ubiquitylating and stabilizing CENP-A in a CAL1-dependent manner. Dev Cell 28, 508-519, doi:10.1016/j.devcel.2014.01.031 (2014).
Personal qualifications:
The ideal candidate for this PhD position is a highly motivated and interactive person with a strong background in cell biology and molecular biology, a strong interest in chromatin biology and epigenetic processes and enjoys being part of an international and collaborative team

How to apply:
Only via the online portal, please refer to Erhardt0216
Send application to
Applications are handled by the Graduate School on campus. All applications must be submitted via the online system and refer to project no: Erhardt0116
A pre-check of materials or eligibilty is not possible.

The Hartmut Hoffmann-Berling International Graduate School of Molecular and Cellular Biology (HBIGS)
Heidelberg University
Im Neuenheimer Feld 501 (HBIGS Center), 1st floor
69120 Heidelberg
T: +49 6221-546720
F: +49 6221-546718
While applying for the job please refer to jobvector and use the following reference number: Erhardt0216

About Universität Heidelberg - ZMBH

The primary goal of the research in my group is to elucidate how epigenetic mechanisms are linked to centromere identity, chromosome segregation, and cell cycle progression. We use diverse cell biological, biochemical, molecular and genetic tools in the fruit fly Drosophila melanogaster and human cell culture for our studies. We have identified regulators that functionally or physically...

More about Universität Heidelberg - ZMBH

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