2 Phd Stellen ab dem 01.01.2019, 65% E13

Project supervisor:
Heineke, Jörg

Application deadline:
31. 1. 2019

Start of PhD project:
As soon as possible

Project description:
Title:
Secreted long non-coding RNAs in the crosstalk between endothelial cells and cardiomyocytes in heart failure

Summary:
We will use OMICS approaches to identify secreted endothelial long non-coding RNAs with impact on cardiomyocyte growth and function during hypertrophic heart disease. Detailed molecular mechanisms will be deciphered using primary cell-culture and co-culture approaches, endothelial and cardiomyocyte specific knock-out mice.

References:
1. Malek Mohammadi M, Kattih B, Grund A, Froese N, Korf-Klingebiel M, Gigina A, Schrameck U, Rudat C, Liang Q, Kispert A, Wollert KC, Bauersachs J, Heineke J. The transcription factor GATA4 promotes myocardial regeneration in neonatal mice. EMBO Mol Med. 2017 Feb;9(2):265-279. doi: 10.15252/emmm.201606602

2. Appari M*, Breitbart A*, Brandes F, Szaroszyk M, Froese N, Korf-Klingebiel M, Mohammadi MM, Grund A, Scharf GM, Wang H, Zwadlo C, Fraccarollo D, Schrameck U, Nemer M, Wong GW, Katus HA, Wollert KC, Müller OJ, Bauersachs J, Heineke J. C1q-TNF-Related Protein-9 Promotes Cardiac Hypertrophy and Failure. Circ Res. 2017 Jan 6;120(1):66-77.

3. Heineke J, Molkentin JD. (2006). Regulation of cardiac hypertrophy by intracellular signalling pathways. Nat Rev Mol Cell Biol 7, 589-600.

Methods that will be used:
Experimental mouse work using heart failure mouse models, primary cardiac endothelial cell and cardiomyocyte isolation and culture, RNA-Sequencing, Proteomics, Isolation of Extracellular vesicles. Molecular Biology. Biochemistry and Histology Methods. Basic Bioinformatics.

Cooperation partners:
Prof. Dobreva, Prof. Wieland, Prof. Goerdt, Prof. Bieback.

Personal qualifications:
We are looking for highly motivated and ambitious candidates with interest in cardiovascular biology holding a Master degree in Biochemistry, Biology or related fields. Solid theoretical and practical knowledge of molecular, biochemical and cell culture models is required. Experience in mouse work and a FELASA degree are an advantage. We offer an excellent scientific environment, inspiring teamwork, close mentoring and a great start of your scientific career.

Keywords:
Cardiovascular Biology and Disease, long non-coding RNA, intercellular cross talk. Molecular Mechanisms of Disease.

Activities and responsibilities

Molecular biology: DNA cloning, qPCR, transcriptome analysis, promoter analysis culture of cardiac muscle and endothelial cells, protein biochemical working methods (immunostaining, Western blots, IPs) Identification of new coding and non-coding RNA as potential future therapeutic approaches Investigation of the communication between endothelial cells and heart muscle cells in heart disease

Qualification profile

Completed degree in biology or biochemistry FELASA Schein, experience in animal experimental work In-depth knowledge and practical experience in molecular, cell and protein biochemical working methods High motivation and resilience, reliability, willingness to take responsibility, willingness to learn, ability to work in a team Analytical and goal-oriented approach, good English and computer

Benefits

An open-minded and motivated team An academic environment that offers its scientific services to support the reconciliation of work and family life for the University of Heidelberg's internal education program

Send application to

Natascha Dorn (Hemmerich)
Sekretariat Herz-Kreislaufforschung
_________________________________________________________________________
Abteilung für Herz-Kreislaufforschung, European Center for Angioscience
Medizinische Fakultät Mannheim der Universität Heidelberg
Ludolf-Krehl-Straße 7-11
Tridomus, Haus B, Ebene 4
D-68167 Mannheim
Tel.: +49 (0) 621 383-71850

While applying for the job please refer to jobvector

About Universitätsklinikum Mannheim der Universitätsklinik Heidelberg

Summary: We will use OMICS approaches to identify secreted endothelial long non-coding RNAs with impact on cardiomyocyte growth and function during hypertrophic heart disease. Detailed molecular mechanisms will be deciphered using primary cell-culture and co-culture approaches, endothelial and cardiomyocyte specific knock-out mice.  

More about Universitätsklinikum Mannheim der Universitätsklinik Heidelberg